How to adjust mobile phase gradient ?

hello,

I reran a vendor's method for identity and purity of a polypeptide. Invendor's method, they use Agilent HPLC. However, I used Perkin Elmer HPLC. I follow the vendor's method exactly: the gradient, column type, column temperature, injection volume,etc. My system suit failed. I think the failure maybe due to the gradient since different instrument has different dwell volume. So, I went on and measured the dwell volume of my instrument. I don't know how to adjust the gradient if the dwell volume is known.



Please help.
Thanks!

I didn't find the right solution from the Internet.


References:

www.chromforum.org/viewtopic.php?f=1&t=25523#p121471



motion graphics examples