Dilution factor when calculating BSA concentration !

hello,

Using stock BSA solution of unknown concentration, I prepared a diluted BSA solution. 50μL of stock BSA and 4950μL of distilled water. I then prepared a cuvette containing 1mL of my dilute BSA solution and 2mL of Bradford reagent. The absorbance was measured and concentration of BSA was calculated to be 42.42 μg/mL (by solving for x from the equation of the line of my known protein calibration curves.)

Thanks!


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